Upstream Bioprocessing

The upstream process is defined as the entire process from early cell isolation and cultivation, to cell banking and culture expansion of the cells until final harvest (termination of the culture and collection of the live cell batch).

Steps of upstream processing are:

Step 1

#1: Cell Isolation and Cultivation

Cells are isolated from their natural environment and grown in a controlled lab setting to ensure healthy cell proliferation. This is the foundation of upstream processing, where cell type selection (mammalian, bacterial, insect, etc.) plays a critical role....

Cell Isolation Cell Cultivation Cell Types: - Mammalian - Bacterial - Insect Upstream Bioprocessing
Step 2

#2: Inoculum and Media Development

Cells are expanded to create a working inoculum for production. Simultaneously, the culture media is optimized to provide the necessary nutrients, pH, and growth conditions for enhanced cell growth and product yield....

Inoculum Expansion Media Optimization Key Factors: - Nutrients - pH Levels - Growth Conditions Upstream Bioprocessing
Step 3

#3: Bioreactor/Fermenter Cultivation

Cells are transferred into bioreactors or fermenters, where they grow under carefully controlled conditions such as oxygen levels, temperature, and shear force. This scale-up process ensures that cells continue to thrive in large volumes while maintaining productivity....

Bioreactor Transfer Controlled Conditions Scale-Up Factors: - Oxygen Levels - Temperature - Shear Force Upstream Bioprocessing
Step 4

#4: Cell Expansion

Cells are expanded progressively in larger vessels to maintain consistent growth and viability as the culture volume increases. The process ensures stable cell health and consistent output throughout the scale-up....

Progressive Expansion Larger Vessels Process Benefits: - Consistent Growth - Viability - Stable Output Upstream Bioprocessing
Step 5

#5: Final Harvest

Once the cells have reached the desired growth stage, they are harvested for downstream processing. This marks the completion of the upstream phase and the preparation of live cells for further product development....

Harvesting Cells Downstream Processing Key Points: - Desired Growth Stage - Preparation for Product Development Upstream Bioprocessing

Applications of upstreams:

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